7. Process of Genetic Modification

Was tasked to find out about genetic modification
and these were what i have found on the web.

Production of GMOs is a multistage process which can be summarized as follows:
1. identification of the gene interest;
2. isolation of the gene of interest;
3. amplifying the gene to produce many copies;
4. associating the gene with an appropriate promoter and poly A sequence and insertion into plasmids;
5. multiplying the plasmid in bacteria and recovering the cloned construct for injection;
6. transference of the construct into the recipient tissue, usually fertilized eggs;
7. integration of gene into recipient genome;
8. expression of gene in recipient genome; and
9. inheritance of gene through further generations.

Reference:
A.Beardmore John, S. Porter Joanne. University of Wales SwanseaUnited Kingdom. Genetically Modified Organisms and Aquaculture. Page 4.
ftp://ftp.fao.org/docrep/fao/006/y4955e/Y4955E00.pdf